|
|
|
|
|
| Carvalho,Rafael Augusto de; Sanchez,Sebástian; Swarça,Ana Claudia; Fenocchio,Alberto Sergio; Martins-Santos,Isabel C.; Dias,Ana Lúcia. |
| This study presents chromosomal data of Megalonema platanum from rio Tibagi, Paraná, Brazil and from rio Paraná, Argentina. The diploid number was equal 54 with karyotype composition of 24m+16sm+2st+12a in both populations. The AgNOR sites were detected in the terminal position of a submetacentric pair of the two analyzed populations, coinciding with secondary constrictions on the short arm of pair 15. CMA3 and FISH with 18S rDNA probe displayed fluorescent signals that correspond to the AgNOR sites and secondary constriction. The presence of a small acrocentric supernumerary chromosome can be observed in M. platanum from rio Tibagi, with centromeric heterochromatin. Others heterochromatic blocks were evidenced in the terminal position of some chromosome... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytogenetics; C-banding; CMA3; FISH; NORs; Pimelodidae. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000100017 |
| |
|
|
| Pires,Larissa Bettin; Usso,Mariana Campaner; Giuliano-Caetano,Lucia; Dias,Ana Lúcia. |
| Abstract The genera Cichlasoma and Gymnogeophagus belong to the subfamily Cichlinae, the only one in Neotropical cichlids. Cichlasoma dimerus, C. paranaense, C. portalegrense, Gymnogeophagus rhabdotus, and G. lacustris were collected at different points in the Paranapanema and Paraguay basins and the Lagoon of Patos hydrographic system. In addition to conventional analysis, CMA3 fluorochrome staining, and FISH with 18S rDNA probe were performed. All species had a diploid number equal to 48, with interand intraspecific differences in karyotype formulae. All species presented a single AgNOR site, except G. rhabdotus and the C. paranaense population of the Paranapanema River, which revealed more than one pair of nucleolar chromosomes. AgNORs were coincident... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome banding; Fish cytogenetics; Karyotype diversification; Ribosomal DNA. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400203 |
| |
|
| |
|
|
| Treco,Fernando Rodrigo; Malabarba,Luiz R.; Giuliano-Caetano,Lucia; Dias,Ana Lúcia. |
| A cytogenetic study was conducted on specimens of Parapimelodus nigribarbis and Pimelodus maculatus collected in the lago Guaíba drainage, Porto Alegre, Rio Grande do Sul, Brazil. The two species had a diploid number of 56 chromosomes, with P. nigribarbis showing a karyotype of 20m + 20sm + 4st + 12a with FN of 100, and P. maculatus showing a karyotype of 24m + 20sm + 6st + 6a with FN of 106. NORs were demonstrated in both species in only one pair of subtelocentric chromosomes, in the terminal region of the long arm, which was coincident with C- banding and CMA3 staining, while DAPI staining was negative in these regions. Parapimelodus nigribarbis had a greater number of heterochromatic bands than did P. maculatus, which were distributed mainly in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parapimelodus; Pimelodus; NOR; Heterochromatin; Fluorochromes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000100010 |
| |
|
| |
|
|
| Matsumoto,Silvia Tamie; Mantovani,Mário Sérgio; Malaguttii,Mirtis Irene Ariza; Dias,Ana Lúcia; Fonseca,Inês Cristina; Marin-Morales,Maria Aparecida. |
| Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa; Chromium; Chromosomes aberrations; Comet assay; Micronucleus; Oreochromis niloticus. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100028 |
| |
|
| |
|
| |
|
|
| Noleto,Rafael Bueno; Kantek,Daniel Luis Zanella; Swarça,Ana Cláudia; Dias,Ana Lúcia; Fenocchio,Alberto Sergio; Cestari,Marta Margarete. |
| We present the karyotypic characterization of 26 specimens of the side-necked turtle Hydromedusa tectifera collected in the upper Iguaçu River, Paraná state, Brazil. The turtles were cytogenetically analyzed using Giemsa staining and other banding techniques (C, G, Ag-NOR and CMA3) as well as fluorescence in situ hybridization (FISH) with a rDNA 18S probe. All the specimens showed a diploid number of 58 composed of 22 macro and 36 microchromosomes. The Ag-NOR, CMA3 and FISH techniques permitted the identification and characterization of the chromosome pairs bearing nucleolus organizer regions (NORs), while G-banding facilitated a better recognition and pairing of macrochromosomes. These data agree with some information available in the literature and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosomes; FISH; G-banding; Pleurodira; Turtle. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200011 |
| |
|
|
|
